45 - 食品科学技术页

的细胞增殖及脂肪代谢的影响 [ J]. 中国食品学报,
2012, 12(10): 10 - 15.
[4] 摇 Frost S C, Lane M D. Evidence for the involvement of
vicinal sulfhydryl groups in insulin鄄activated hexose trans鄄
port by 3T3 - L1 adipocytes [ J ]. Journal of Biological
Chemistry, 1985, 260(5): 2646 - 2652.
[5]摇 陈思凡, 肖新才 , 孙延双, 等. 白藜芦醇对小鼠 3T3-
L1 细胞增殖与分化的影响及其机制 [J]. 中国药理学
通报, 2010, 26(1): 108 - 111.
[6]摇 刘芳芳, 杨明炜, 王晓强, 等. 梓醇与小檗碱及其配
伍对胰岛素抵抗 3T3- L1 脂肪细胞的影响 [ J]. 中草
药, 2007, 38(10): 1523.
[7]摇 郭晓农, 张汝学, 贾正平, 等. 地黄寡糖对 3T3-L1 脂
肪细胞增殖及胰岛素抵抗的作用[J]. 中国中药杂志,
2006, 31(5): 403 - 407.
[8]摇 Student A K, Hsu R Y, Lane M D. Induction of fatty
acid synthetase synthesis in differentiating 3T3-L1 pread鄄
ipocytes[J]. Journal of Biological Chemistry, 1980, 255
(10): 4745 - 4750.
[9]摇 Ramirez鄄Zacarias J L, Castro鄄Munozledo F, Kuri鄄Har鄄
cuch W. Quantitation of adipose conversion and triglycer鄄
ides by staining intracytoplasmic lipids with oil red O
[J]. Histochemistry, 1992, 97(6): 493 - 497.
[10]摇 皮荣标, 林穗珍. 3鄄异丁基鄄1鄄甲基黄嘌呤对低钾诱导的
大鼠小脑颗粒神经元凋亡的 Ca2 + / cAMP 不依赖性保护
作用[J]. 中国药学杂志, 2001, 36(4): 240 -244.
[11]摇 杨夏, 彭生, 刘功俭. cAMP / PKA / CREB 信号通路及
相关调控蛋白 PDE4 和 ERK 对学习记忆的影响[J].
医学综述, 2011, 17(5): 2241.
[12]摇 Park S J, Ahmad F, Philp A, et al. Resveratrol amelio鄄
rates aging鄄related metabolic phenotypes by inhibiting
cAMPphosphodiesterases [ J ]. Cell, 2012, 148 ( 3 ):
421 - 433.
[13] 摇 鞠大鹏, 詹丽杏. 脂肪细胞分化及其调控的研究进
展[J]. 中国细胞生物学学报, 2010(5): 690 - 695.
Optimization of Induction Condition of 3T3-L1
Preadipocytes Differentiation
WANG Yousheng,摇 TIAN Yuanyuan,摇 CAI Qiwei
( School of Food and Chemical Engineering / Beijing Engineering and Technology Research Center of Food Additives /
Beijing Laboratory for Food Quality and Safety, Beijing Technology and
Business University, Beijing 100048, China)
Abstract: The present study investigated the influences of the concentration of 3鄄isobutyl鄄1鄄methylxan鄄
thine (IBMX), dexamethasone and insulin during the combined induction stage ( first stage) and the
corresponding induction time, the induction time and concentration of insulin during the differentiation
stage ( second stage) on 3T3 - L1 preadipocytes differentiation using L 18 (37 ) orthogonal experiment.
Range analyses showed that the impacts of those factors on results were ( from high to low): IBMX,
induction time of the first stage, insulin concentration during the second stage, insulin concentration dur鄄
ing the first stage, dexamethasone concentration and induction time of the second stage. Analysis of vari鄄
ance showed that IBMX played a highly significant influence on the process of 3T3-L1 preadipocytes dif鄄
ferentiation, and the influence of induction time of the first stage and insulin concentration during the sec鄄
ond stage were significant. In contrast, no significant difference was observed in the treatments of dexam鄄
ethasone and insulin concentration. The optimal induction condition for differentiation of 3T3-L1 preadi鄄
pocytes was presented as following: 500 - 750 滋mol / L IBMX, 0郾 25 - 1 滋mol / L dexamethasone, and
1 滋g / mL insulin for 72 h, and then 5 - 10 滋g / mL insulin for 48 h. The result of red oil O staining
showed that the differentiation rate of 3T3-L1 preadipocytes of the presented optimal condition was higher
than that of the rest.
Key words: 3T3-L1; IBMX; insulin; induction
( 责任编辑: 叶红波)
24 食品科学技术学报 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 2014 年 5 月