50 - 食品科学技术页

图 9摇 纤维素酶系活力比较
Fig. 9摇 Compared assay of cellulase activity
由图 9 可以看出, 解淀粉芽孢杆菌来源 CM鄄
Case、FPase、 茁鄄Gase、C1 别为 307郾 23,70郾 37,131郾 55,
16郾 39 U / mL. 除微晶纤维素酶活力稍弱外, 其余 3
种酶活力均相对较高.
3摇 结摇 论
选育了一株具有潜在应用于饲料添加剂的产纤
维素酶菌株 B16,经鉴定为解淀粉芽孢杆菌, 其较适
反应温度为 30 益,较适 pH 为 8郾 0. 该酶在 10 ~ 60
益 稳定性较高 ,60 益 下保温 60 min 后相对酶活性为
97郾 47% . 在 pH 5 ~ 8 稳定性较高,pH 8 体系中保温
60 min 后还有 93郾 81% 的酶活性. Mn2 + 和 Ba 2 + 是酶
的激活因子,Fe 2 + 和 Co2 + 是酶的抑制因子. 除微晶
纤维素酶活力稍弱外,其余酶活力均相对较高 ,CM鄄
Case、FPase、 茁 鄄Gase、C1 活力分别为 307郾 23,70郾 37,
131郾 55,16郾 39 U / mL. 研究结果表明,B16 菌株代谢
产生的纤维素酶在饲料添加剂方面具有应用潜力.
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第 32 卷 第 3 期摇 摇 摇 摇 摇 摇 摇 崔海洋等: 产纤维素酶的解淀粉芽孢杆菌分离鉴定及酶学性质研究