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Comparative Study of Different Materials Immobilized Naringinase
DUAN Zhitao 1 ,摇 XIA Jinlan2 ,摇 SHAN Yang1,3,*
(1 . Longping Branch Graduate School, Central South University , Changsha 410125, China;
2 . School of Resource Process and Bioengineering, Central South University , Changsha 410083, China ;
3. Citrus Resources Comprehensive Utilization of Local and National Joint Project Laboratory /
Hunan Agricultural Product Processing Institute, Changsha 410125, China )
Abstract: Chitosan, sodium alginate, activated carbon,and graphene oxide were selected to immobilize鄄
naringinase. By comparing the activities of immobilized naringinase and the enzyme鄄carrying rates of the
enzyme carriers, it was found that graphene oxide was the optimal material. The enzyme鄄carrying rate of
graphene oxide was 85郾 61% and theenzyme activity of graphene oxide immobilized naringinase was
410郾 50 U / g. The enzyme activity remained 72郾 38% relative activity after 7 times using. Simultaneously,
the effects of different conditions on catalytic activity of graphene oxide immobilized naringinase were
studied. It was obtained that the optimal load amount of naringinase per gram of graphene oxide 60 mg,
optimal temperature 20 益, optimal pH 4郾 0, and immobilization adsorption time 9 h. The activity of gra鄄
phene oxide immobilized naringinase reached 433郾 70 U / g under the optimized conditions, which re鄄
mained 78郾 62% relativity activity after 7 times using.
Key words: chitosan; sodium alginate; activated carbon; graphene oxide; immobilization; naringinase
( 责任编辑: 李摇 宁)
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