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Isolation and Identification of Endophytes in Wild Noni
Leaves from Paracel Islands
BAI Feirong1 , 摇 LIU Yang 1,* , 摇 CAO Yanhua1 , 摇 YAO Su1 ,摇 LI Jinxia 1
TAN Wangqiao2 ,摇 CHENG Chi 1,*
(1郾 China National Research Institute of Food and Fermentation Industies ,China Center of
Industrial Culture Collection, Beijing 100015,China;
2郾 Hainan Noni Biological Engineering Development Co. Ltd. , Haikou 570125, China )
Abstract: The diversity of culturable endophytes in wild Noni leaves from Paracel Islands was discussed
in this study. Strains were isolated by using conventional tablet separation methods. The isolates were
identified by the fungal internal transcribed spacer 1 and 2 ( ITS gene) amplification and the bacterial
16S rDNA amplification, combined with phylogenetic analysis. Total 32 endophytes were isolated and
identified as 19 types. Two types of mold were Chaetomium sp. and Nemania sp. Seventeen types of bac鄄
teria (30 strains) belonged to 7 genera Bacillus sp. , which were Lysinibacillus sp. , Micrococcus sp. ,
Terribacillus sp. , Enterobacter sp. , Xanthomonas sp. , Flavobacterium sp. , and Bacillus sp. . Bacillus
sp. was the dominant genus among the endophytic bacte, which had 20 strains divided into 11 types.
Key words: Noni leaves from Paracel islands; endophytic bacteria; community diversity
( 责任编辑: 李摇 宁)
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第 33 卷 第 1 期摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 白飞荣等: 西沙野生诺尼叶片内生菌的分离与初步鉴定