44 - 食品科学技术学报页

见, 该模型能够较为准确地反映出三种因素对于苦
荞麦粉中的黄酮提取率的影响.
3摇 结摇 论
利用乙醇 -硫酸铵双水相微波辅助提取苦荞麦
粉中黄酮类化合物的较优条件为萃取剂是体积分数
为 30% 的乙醇溶液,微波功率 550 W,微波提取时间
70 s,料液比为 1颐 50 ( g / mL), 苦荞麦粉中黄酮类化
合物提取率为 13郾 82 mg / g. 而苦荞麦粉中苦荞麦黄
酮含量约占总重量的 2郾 31% [11] , 利用乙醇- 硫酸铵
双水相微波辅助提取苦荞麦粉中黄酮的得率约为
1郾 38% ,占总黄酮量的 59郾 7% ,得率较高,操作不复
杂, 用时较短,具有较强的可操作性. 由此可得乙醇
-硫酸铵双水相微波辅助提取适合于苦荞麦粉中黄
酮类化合物的提取.
参考文献:
[1] 摇 林汝法. 中国荞麦 [ M]. 北京: 中国农业出版社 ,
1994:1 - 2.
[2]摇 Stojilkovski K, Ko 姚cevar N G, Samo K, et al. Fagopyrin
and flavonoid contents in common, Tartary, and cymo鄄
sumbuckwheat[ J]. Journal of Food Composition and A鄄
nalysis,2013,32:126 - 130.
[3]摇 施卫省,罗小林, 曹新佳. 苦荞麦黄酮含量萃取工艺的
研究[J]. 中国农学通报, 2012, 28(15): 260 - 263.
[4] 摇 Lee Chiachen, Shen Siouru, Lai Yingjang. Rutin and
quercetin, bioactive compounds from tartary buckwheat,
prevent liver inflammatory injury [ J]. Food Funct,2013
(4):794 - 802.
[5]摇 王红育, 李颖. 荞麦的研究现状及应用前景 [ J]. 食品
科学,2004,25(10):388 - 391.
[6]摇 徐春明,李丹 . 响应面法优化微波辅助提取紫苏中迷
迭香酸[J]. 北京工商大学学报: 自然科学版 , 2012,
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[7]摇 王志华,马会民, 马泉莉, 等. 双水相萃取体系的研究
[J]. 应用化学,2001,18(3):173 - 175.
[8]摇 林金清, 董军芳, 李夏兰. 乙醇 / 硫酸铵双水相体系萃
取甘草酸钾的研究[J]. 精细化工,2004,21(3):165 -
173.
[9]摇 程俊丽,田晋, 冯翠萍. 荞麦粉中黄酮类化合物提取工
艺的研究[J]. 农产品加工·学刊,2009(5):33 - 36.
[10]摇 孙彦. 生物分离工程 [M]. 北京 : 化学工业出版社 ,
2010: 116 - 122.
[11]摇 赵玉平,肖春玲. 苦荞麦不同器官总黄酮含量测定及
分析[J]. 食品科学,2004,25(10):264 - 266.
Optimization of Microwave鄄assisted Extraction Process for
Total Flavonoid from Tartary Buckwheat Flour
XU Chunming,摇 LI Ting,摇 WANG Yingying,摇 PANG Gaoyang,摇 LIU Ying
( School of Food and Chemical Engineering / Beijing Higher Institution Engineering Research Center of Food
Additives and Ingredients / Beijing Key Laboratory of Food Flavor Chemistry,
Beijing Technology and Business University, Beijing100048, China)
Abstract: Flavonoids were extracted from buckwheat powder by the microwave鄄assisted ethanol鄄ammoni鄄
um sulfate aqueous two鄄phase system. The optimal extraction system of flavonoids from tartary buckwheat
powder was determined through single factor experiments and the optimal concentration of ethanol and am鄄
monium sulfate dosage were obtained. The results showed that the method of microwave assisted aqueous
two鄄phase extraction was suitable for flavonoids extraction from tartary buckwheat powder. The extraction
conditions were optimized by the response surface method and the optimized conditions were 30% ethanol
as extraction agent, the microwave power 550 W, extraction time 70 s, and the material鄄to鄄liquid ratio
0郾 02 g / mL. The yield of flavonoids was 1郾 38% under the optimized conditions.
Key words: aqueous two鄄phase; microwave assisted extraction; response surface method; tartary buck鄄
wheat powder; flavonoids
( 责任编辑: 李摇 宁)
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第 32 卷 第 6 期摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 徐春明等: 微波辅助双水相提取苦荞麦粉中黄酮类化合物