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Effect of Different pH on Activity and Conformation of Ana鄄rLOX
WANG Xiaoming,摇 ZHU Xiaoyu,摇 ZHANG Chong,摇 L譈 Fengxia,摇 BIE Xiaomei,摇 LU Zhaoxin
*
(
College of Food Science and Technology
,
Nanjing Agricultural University
,
Nanjing
210095,
China
)
Abstract
: The conformations of
Anabaena
Sp. lipoxygenase ( ana鄄rLOX), such as secondary structure,
helical structure, turn structure, and disordered structure were studied in different pH buffers. The corre鄄
lation between the activity and conformation of ana鄄rLOX was studied. The results showed that the optimal
pH and the most stable pH of ana鄄rLOX were 9 and 7. The enzyme activity was correlated with the con鄄
tent of helical structure, turn structure, and disordered structure. The enzyme activity positively correla鄄
ted with the helical structure and turn structure while the enzyme activity negatively correlated with the
disordered structure under the pH value between 6 and 9. The enzyme was most stable when the contents
of helix, sheet, turn, and random structures were 20% - 30% .
Key words
: lipoxygenase; enzyme activity; secondary structure; buffer solution
(
责任编辑
:
叶红波
)
12
第
33
卷 第
4
期
摇 摇 摇 摇 摇 摇 摇 摇 摇
汪晓鸣等
:
酸碱度对重组鱼腥藻脂肪氧合酶构象与活性的影响
