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Electrochemical Immunosensor for Diethylstilbestrol Determination Based on
Graphene鄄Chitosan Composite Modified Glassy Carbon Electrode
HUANG Shengquan
1
,摇 FU Meng
1
,摇 ZHANG Jie
2
,摇 LI Wenzhi
1
,摇 ZHANG Qin
2
,摇 SHAO Kefeng
2
,摇 ZHAO Bo
2,*
(1
. Infinitus
(
China
)
Co Ltd
,
Guangzhou
510030,
China
;
2
. School of Chemistry and Materials Science
,
Nanjing Normal University
,
Nanjing
210023,
China
)
Abstract
: A graphene鄄chitosan modified glassy carbon electrode was prepared. Electrical conductivity of
the modified glassy carbon electrode (GCE) was investigated by cyclic voltammetry (CV) . Competitive鄄
electrochemical immunoassay was performed to detect diethylstilbestrol by differential pulse voltammetry
(DPV) . Under the optimized conditions, the 驻鄄value of oxidation peak current was proportional to
diethylstilbestrol concentration in the range between 1 and 2 500 滋g / L. The linear equation was 驻
I
=
25郾 036 8 + 0郾 003 42
籽
(
R
= 0郾 991 24), and the detection limit was 0郾 1 滋g / L. A good recovery of dieth鄄
ylstilbestrol in the range of 93郾 2% - 106郾 8% was obtained in pork, beef, duck, and milk powder.
Key words
: diethylstilbestrol; graphene; chitosan; differential pulse voltammetry
(
责任编辑
:
李
摇
宁
)
17
第
34
卷 第
1
期
摇 摇 摇 摇 摇 摇 摇 摇 摇
黄生权等
:
石墨烯
-
壳聚糖复合材料修饰电极检测己烯雌酚
