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Optimization on Biotransformation of Quercetin Glycosides in

Rosa roxburghii by 茁鄄Glucosidase

*
YU Yihong,摇 GU Yuanting,摇 DING Zhuhong ,摇 CHEN Siqi,摇 SONG Yuting,摇 WANG Yi
(School of Liquor & Food Engineering, Guizhou University / Rosa Roxburghii Engineering Technology Research
Center, National Forestry and Grassland Administration / Key Laboratory of Agricultural and Animal Products
Store and Processing of Guizhou Province, Guiyang 550025, China)

Abstract: To explore the improvement of biotransformation approach of Rosa roxburghii flavonoid aglycon
releasing ability, 茁鄄glucosidase from different sources were used to hydrolyze quercetin鄄3鄄O鄄rutinoside,
quercetin鄄3鄄O鄄rhamnoside, and quercetin鄄3鄄O鄄glucoside. Quercetin content and glycoside conversion rate
as indicators, the conversion rate of quercetin glycosides and content of quercetin hydrolyzed by 茁鄄
glucosidase from Lactobacillus acidophilus, Trichoderma, and almond were dynamically monitored by
HPLC. And the optimized enzymatic hydrolysis time, enzymatic hydrolysis pH, enzymatic hydrolysis
temperature, and enzyme dosage (enzyme鄄substrate mass ratio) were chose as single factors, and the
effects of independent change of each factor on the index were examined. Then Box鄄Behnken method was
used to study the influence of each factor and its interaction on the conversion rate, and to optimize the
process conditions. Almond 茁鄄glucosidase hydrolyzed the three glycosides and converted them to the
highest quercetin content. The conversion rates for different substrates were in the order of quercetin鄄3鄄O鄄
glucoside(74郾 10% ), quercetin鄄3鄄O鄄rutinoside(64郾 30% ), and quercetin鄄3鄄O鄄rhamnoside (31郾 80% ).
The optimal optimized hydrolysis process conditions of almond 茁鄄glucosidase were hydrolysis time of
28郾 90 min, hydrolysis pH value of 4郾 9, hydrolysis temperature of 52 益, and the enzyme dosage of
0郾 08% . Under these conditions, the conversion rate of quercetin鄄3鄄O鄄rutinoside, quercetin鄄3鄄O鄄
rhamnoside, and quercetin鄄3鄄O鄄glucoside were 71郾 48% , 36郾 32% , and 77郾 86% , respectively.
Keywords: Rosa roxburghii; quercetin glycoside; quercetin; 茁鄄glucosidase; biotransformation
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