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Effects of Dissolved Oxygen on Biosynthesis of Orange
Pigments in Monascus Fermentation
ZHANG Huijuan1 ,摇 ZHU Xiaogang2 ,摇 MAO Peng3 ,摇 NIU Guoqiang 3 ,摇 ZHAO Jie2 ,摇 XU Ganrong 3,*
(1 . Shenzhen Main Luck Pharmaceuticals Inc. , Shenzhen, 518029, China ;
2 . Liangshan Chia Tai Ling Hua Bio鄄Tech Co. Ltd. , Jining 272600, China;
3 . School of Biotechnology , Jiangnan University, Wuxi 214122, China )
Abstract: Effect of dissolved oxygen (DO) on the biosynthesis of orange pigments in Monascus fermenta鄄
tion was investigated in this study. The orange pigments production of the w1 Xia strain in a 10 L bioreac鄄
tor under different DO levels obtained using different ventilatory volumes and stirring speeds were stud鄄
ied. And the optimized conditions were applied in a 50 L bioreactor. The results showed that the chan鄄
ging trend of substrate consumption, pH, and DO was similar under different ventilatory volume condi鄄
tions, but the color value of orange pigments increased along with the ventilatory volume augment. The
stirring speed did not have significantly effects on the biomass and color value of orange pigments. Mean鄄
while, the orange pigments production in a 50 L bioreactor was similar to that in a bioreactor. In conclu鄄
sion, the higher ventilatory volume and adapted stirring speed were beneficial to the biomass and orange
pigments production.
Key words: Monascus; orange pigment; fermentation conditions; color value; dissolved oxygen
( 责任编辑:叶红波)
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第 33 卷 第 6 期摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 张慧娟等: 溶氧对红曲菌发酵生物合成橙色素的影响