Page 82 - 《食品科学技术学报》2020年第5期

第 38 卷 第 5 期摇 摇 摇 摇 摇 摇 摇 何大博等: 利用酪蛋白酸钠的分子伴侣特性抑制蛋清蛋白的热聚集研究 7 7

illustrated that the inhibitory effect of sodium caseinate on the thermal aggregation of ovalbumin and
ovaltransferrin determined its effect on preventing thermal aggregation the egg white proteins. With the
increase of the addition of sodium caseinate, the insoluble aggregates of egg white proteins was gradually
changed to soluble aggregates, and the large and dense aggregates were transformed to small and loose.
The results indicated that sodium caseinate could prevent the thermal aggregation of egg white proteins.
Keywords: sodium caseinate; egg white protein; thermal aggregation; chaperone; thermal stability;
inhibitory effect

(责任编辑:檀彩莲)
詬詬詬詬詬詬詬詬詬詬詬詬詬詬詬詬詬詬詬詬詬詬詬詬詬詬詬詬詬詬詬詬詬詬詬詬詬詬詬詬詬詬詬詬詬詬詬
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Study on Lipid鄄Lowering Mechanism of Chitooligosaccharides on

Non鄄Alcoholic Fatty Liver Cell Model

*
SHEN Xin,摇 ZHAO Mengyao,摇 QIU Yongjun,摇 XIA Quanming,摇 ZHAO Liming
(School of Bioengineering, East China University of Science and Technology, Shanghai 200237, China)

Abstract: To investigate the effect and potential mechanism of chitooligosaccharides on reducing liver
lipid accumulation in vitro, sodium oleate was used to induce HepG2 cells to establish non鄄alcoholic fatty
liver cell model, the lipid profiles including total lipid accumulation, total triglyceride, total cholesterol,
high / low density lipoprotein cholesterol and free fatty acids contents were analyzed by oil red O staining
and corresponding commercial kits, respectively. The expression of genes and proteins related to
intracellular lipid metabolism were detected by real鄄time PCR and western blot. The results indicated that
4郾 0 mg / mL chitooligosaccharides had a significant inhibitory effect on liver lipid accumulation and
reduced the mRNA and protein expression levels of diacylglycerol acyltransferase 2 (DGAT2), liver X
receptor alpha ( LXR琢 ), peroxisomes proliferator鄄activated receptor鄄酌 ( PPAR酌 ), pregnenolone X
receptor ( PXR ) and differentiation cluster 36 ( CD36 ). These results suggested that
chitooligosaccharides could reduce the accumulation of lipids by regulating fatty acid absorption and
triglyceride synthesis pathways. This study aims to provid theoretical support for using
chitooligosaccharides as dietary supplements in lipid鄄lowering and liver protection.

Keywords: chitooligosaccharides; non鄄alcoholic fatty liver; cell model; lipid鄄lowering mechanism;
CD36; DGAT2

(责任编辑:叶红波)