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第 38 卷 第 5 期摇 摇 摇 摇 摇 摇 摇 摇 刘晓丽等: 壳寡糖-O-曲酸聚合物的理化性质及抗氧化活性研究 3 9

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Studies on Physico鄄Chemical Properties and Antioxidant
Activities of Chitosan Oligosaccharide鄄O鄄Kojic Acid Polymers

LIU Xiaoli,摇 XIA Wenshui
(State Key Laboratory of Food Science and Technology / School of Food Science and
Technology / Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu
Province, Jiangnan University, Wuxi 214122, China)

Abstract: Chitosan oligosaccharide (COS) and kojic acid (KA) were used as synthetic initiators and the
COS -based polymer ( COS - O - KA) were synthesized by selective partial alkylation reaction to graft
kojic acid onto the COS molecular chain. After characterization of structure by NMR spectroscopy,
molecular weight, polydispersity index, water鄄solubility, hemolysis assay in vitro, biocompatibility in

vitro, antioxidant activity, and animal toxicity of COS -O-KA were studied. The results revealed that the
three polymers of different substitution degree (COS -O -KA1-3) were synthesized successfully which
proved by NMR spectroscopy. In addition, COS - O - KA1 - 3 exhibited preferable water鄄solubility,
hemolytic activity in vitro, biocompatibility in vitro, and scavenging activity of DPPH free radical and
+
ABTS free radical in comparison with that of the free COS and KA. Among them, when the