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Determination of Aflatoxin B1 , B2, G1 and G2 in corn by
HPLC with Photochemical Derivation
FENG Qiuyue 1 ,摇 LIU Ying1 ,摇 LIU Yuanyuan1 ,摇 YANG Yunxia2 ,摇 WANG Anru 1,*
(1. State Key Laboratory of Direct鄄Fed Microbial Engineering, Beijing Da Bei Nong Science and Technology
Group Co. Ltd. , Beijing 100192, China;
2. Institute of Quality Standard and Testing Technology for Agro鄄product of CAAS , Beijing 100081, China )
Abstract: A method for the determination of aflatoxin B1,B2,G1,and G2 in corn by high performance
liquid chromatography (HPLC) with immunoaffinity column and photochemical derivatization was estab鄄
lished. Samples were extracted, concentrated, and filtered purified by immunoaffinity column, and afla鄄
toxin B1 and G1 were derivatived using photochemical derivatization unit after the HPLC separation.
Then four aflatoxins B1, B2, G1, and G2 were analyzed by HPLC with fluorescence detector. Results
showed that the peak elution time of four aflatoxins B1, B2, G1, and G2 were in the range of 6 - 11 mi鄄
nutes. Meanwhile, the detection limit of quantitation of four aflatoxins were 0郾 10,0郾 03,0郾 10,and 0郾 03
滋g / kg, which was above the limit of quantitation of AFTs in EU regulation, and the detection limit of
quantitation of B1 and G1 enhanced to 2郾 7 times and 3郾 6 times. The recoveries of four aflatoxins were
90郾 3% ,85郾 6% ,93郾 5% ,and 92郾 8% when the concentration of B1, B2, G1 and G2 were 10,3,10,3
滋g / L. The linearity range of B1,B2,G1,and G2 were 0 - 20,0 - 6,0 - 20,and 0 - 6 滋g / L, and relative
standard deviation were 2郾 3% ,1郾 5% ,2郾 7% ,and 3郾 2% . This method was accurately and sensitively to
analysis four aflatoxins B1,B2,G1,G2 in corn.
Key words: aflatoxins; corn; high performance liquid chromatography; immunoaffinity column; photo鄄
chemical derivatization
( 责任编辑:李摇 宁)
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第 33 卷 第 5 期摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 冯秋月等: 光化学衍生检测玉米中 4 种黄曲霉毒素