3摇
结
摇
论
以无毒无害
、
水溶性好的
1,2鄄
丙二醇为有机溶
剂
,
以含有
85%
以上不饱和脂肪酸的葵花籽油为原
料
,
通过碱异构化反应
,
在适宜的温度和适宜的时间
内合成共轭亚油酸
(CLA),
研究了反应时间
、
反应温
度和碱催化剂用量等
3
个影响
CLA
产率的因素
,
得
出了反应的较优条件为在氮气保护下
,1,2鄄
丙二醇
作为有机溶剂
,
葵花籽油为原料
,
溶剂的质量比
m
(
葵花籽油
) 颐
m
(
溶剂
) = 1颐 3,
反应温度
170 益,
反
应时间为
2郾 5 h,
加碱量与皂化值的质量比为
2郾 2。
以此条件制备的
CLA
质量分数为
47郾 80% ,
且样品
的颜色较浅
,
不浑浊
。
参考文献
:
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high鄄performance liquid chromatographic separation and
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leic acid and atherosclerosis in rabbits[J]. Atherosclero鄄
sis, 1994, 108(1): 19 - 25.
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linoleic acid on body composition in mice [ J]. Lipids,
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[4]摇 Chin S F, Liu W, Storkson J M, et al. Dietary sources of
conjugated dienoic isomers of linoleic acid, a newly rec鄄
ognized class of anticarcinogens[J]. Journal of food com鄄
position and analysis, 1992, 5(3): 185 - 197.
[5]摇
张根旺
,
杨天奎
.
生物活性物质共轭亚油酸
( CLA)
的研究
———CLA
的生理活性和应用前景
[ J].
中国油
脂
, 2000, 25(6): 13 - 16.
[6]摇
张树林
,
庞登甲
,
宫素芝
. C22
三元羧酸的合成与应用
研究
[J].
精细石油化工
,1997(2):37 - 39.
[7]摇 Vijayalakshmi P, Subbarao R, Lakshminarayana G.
Preparation and surface鄄active properties of the sodium
soaps, mono鄄and diethanolamides and diol and triol sul鄄
fates of cycloaliphatic C21 Di鄄and C22 Tricarboxylicacids
[ J ]. Journal of the American Oil Chemists Society,
1991, 68(2): 133 - 137.
[8]摇
李藴
.
共轭亚油酸的合成及在食品中的应用研究
[ J].
科技致富向导
,2012,35(7):13 - 14.
[9]摇
吴冀华
,
裘爱泳
.
气相色谱法分析共轭亚油酸异构体
[J].
中国油脂
,2002,27(1):65 - 67.
[10]摇
黎明
.
相转移催化及其在有机合成中的应用
[M].
长
春
:
东北师范大学出版社
,1987.
[11]摇
刘晓华
,
曹郁生
,
陈燕
.
共轭亚油酸分析方法的研究
进展
[J].
中国油脂
,2004,29(7):48 - 50.
[12]摇
余迎利
,
邓泽元
,
曹树稳
. Ag鄄HPLC
测定共轭亚油酸
的研究
[J].
食品与机械
,2005(6):58 - 59.
[13]摇
关奇
.
葵花籽油制备共轭亚油酸的研究
[D].
北京
:
中
央民族大学
,2006.
Optimization of Process Conditions for Synthesis of Conjugate
Linoleic Acid by Alkali Isomerization
ZHA Mengyin,摇 LU Zhixing,摇 WU Hao,摇 ZHOU Zheng,摇 CHEN Cunshe
*
(
Beijing Higher Institution Engineering Research Center of Food Additives and Ingredients
,
Beijing Technology and Business University
,
Beijing
100048,
China
)
Abstract
: The conjugate linoleic acid (CLA) was prepared by the alkali isomerization with sunflower oil
as raw material. Effects of solvent varieties, reaction time, reaction temperatures, and catalyst dosages on
the yield of CLA were analyzed by the single factor experiment and orthogonal experiment with three fac鄄
tors and three levels. The results showed that optimized conditions were as following, 1,2鄄propylene gly鄄
col as solvent, the ratio of sunflower oil and solvent 1颐 3, the reaction temperature 170 益, the reaction
time 2郾 5 h, and the ratio of alkali and saponification equivalent 2郾 2. The yield of CLA was 47郾 80% un鄄
der the optimized conditions.
Key words
: sunflower oil; conjugated linoleic acid; alkali isomerization method; synthesis
(
责任编辑
:
李
摇
宁
)
66
食品科学技术学报
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摇 2015
年
3
月
