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Effect of Grape Seed Proanthocyanidins on Gut
Microbiota of Diet鄄Induced鄄Obesity Rats
SONG Xuelin
1
,摇 LI Yamei
1
, 摇 XIAO Junsong
1,*
, 摇 WU Hua
2
, 摇 CAO Yanping
1
(1
. Beijing Key Laboratory of Food Flavor Chemistry / Beijing Engineering and Technology Research
Center of Food Additives / Beijing Laboratory for Food Quality and Safety
,
Beijing Technology and
Business University
,
Beijing
100048,
China
;
2
. College of Life Sciences
,
Jianghan University
,
Wuhan
430070,
China
)
Abstract
: Wistar rats were fed with high fat diet to induce the diet鄄induced obesity model (DIO), and
then gavaged with grape seed proanthocyanidins (GSP) of different doses. Culture鄄independent methods
such as denaturing gradient gel electrophoresis (DGGE) and real鄄time fluorescent quantitative polymerase
chain reaction (real鄄time PCR) were applied, coupled with multivariate analysis, to explore the effect of
GSP on the microbiota of DIO rats. Results showed that GSP treatment (100 mg / (kg·bw·d)) could in鄄
hibit high鄄fat induced obesity, and its gut microbiota profile was separated from that of the model group.
Results also suggested that GSP鄄treated groups had the lower
Firmicutes
content and higher
Bacteroidetes
content and the ratio of
Firmicutes
to
Bacteroidetes
increased in this group. Real鄄time鄄PCR results indica鄄
ted that GSP promoted the proliferation of
Bacteriodes spp
. and inhibited that of
Clostridium leptum
. The
key genera the GSP might have an effect on were as follows:
Blautia
,
Bacteroides
,
Lactobacillus
,
Anaero鄄
stipes
,
Clostridium
,
Anaerofilum.
Key words
: grape seed proanthocyanidins; obesity; gut microbiota; denaturing gradient gel electropho鄄
resis; real time鄄PCR
(
责任编辑
:
叶红波
)
64
食品科学技术学报
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摇 2015
年
9
月
