In this study, using the prepared trichlorfon molecularly imprinted polymer as sorbent, a novel method for the determination of trichlorfon residues in grape wine was developed by utilizing molecularly imprinted solid-phase extraction coupled to capillary electrophoresis -CE-. Trichlorfon in the sample was selectively adsorbed onto molecularly imprinted polymer -MIP-. After the sample loading was completed, the molecularly imprinted solid-phase column was eluted with 2mL methanol/acetic acid -90∶10, v/v-. The elution was condensed to dryness under a gentle flow of nitrogen, accurately re-dissolved with 05mL doubly deionized water -DDW-, and then injected into CE for analysis. The limit of detection -LOD- was 75μg/L, and the RSD was 450%. The blank grape wine samples spiked with trichlorfon at three levels were extracted and determined by this presented method with recoveries ranging from 803% to 962%.