In this study, Alcalase was utilized to extract Andrias davidianus oil from the tails of the cultured Chinese giant salamander and the enzymic technological conditions were researched. The orthogonal test was conducted to evaluate the effects of four factors, the reaction temperature, reaction time, enzyme amounts, and pH on the extraction yield of Andrias davidianus oil. The optimal parameters of the extraction process were as follows: the optimal temperature-50℃-, the reaction time -1h-, enzyme amounts -15%-, and pH-6-. Raw Andrias davidianus oil was consistent with the national secondary standards on raw fish oil. 14 kinds of fatty acids were detected in raw Andrias davidianus oil. The saturated fatty acids were about 375% and the unsaturated fatty acids were 625%. The contents of DHA and EPA were 47% and 42%, respectively.