红曲霉M1发酵糙米代谢产物抗S180细胞增殖效应研究
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国家自然科学基金资助项目-31171729-


Studies on Metabolites of Monascus M1 Fermented Brown Rice and Antiproliferation Effect on S180 Ascitic Tumor Cell
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    摘要:

    以红曲霉M1分别发酵大米和糙米,比较其代谢产物的变化;采用MTT比色法,初步研究了Monacolin K标准品、红曲糙米及红曲大米乙醇提取物对S180小鼠腹水瘤细胞的抑制作用. 结果表明:在MBR乙醇提取物中,可检测到MK的酸式构型,MK含量约为MR乙醇提取物的13倍. 经红曲霉M1发酵后,MBR中γ-氨基丁酸-GABA-质量浓度达到298mg/g,是MR乙醇提取物的31倍. MBR中桔霉素质量浓度低于MR. MK标准品、MBR及MR乙醇提取物均对S180小鼠腹水瘤细胞具有一定的抑制作用. MK质量浓度在3~50 μg/mL,三者抑制S180小鼠腹水瘤细胞增殖效应随MK质量浓度的增大而增大. 在MK含量相同的条件下,MR和MBR乙醇提取物的抑制效果显著大于MK标准品-P<001- .

    Abstract:

    The production of Monascus M1 metabolites with rice and brown rice was compared. The antiproliferation effect of Monacolin K -MK- , the ethanol extracts of fermentation product of red yeast brown rice -MBR- and rice -MR- on S180 ascitic tumor cell in vitro was studied with the MTT colorimetric method. It showed that the acid form MK was presented in MBR, and the total output of MK was higher -30%- than that of MR. The content of GABA was 298mg/g in MBR, which was 21-fold higher than that in MR. While the yield of citrinin was lower in MBR. MK alone, ethanol extracts from MR and MBR were all active in inhibiting S180 ascitic tumor cell growth. In a certain range of MK concentration -3~50μg/mL-, the capacity of antiproliferation increased gradually with the increase of the concentration. When the concentration of MK was equal, the inhibition effect of MBR and MR was significantly stronger than MK alone -p<001- .

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王 婧,王昌禄,陈勉华,杨 华,李素珍,王玉荣,李贞景.红曲霉M1发酵糙米代谢产物抗S180细胞增殖效应研究[J].食品科学技术学报,2013,31(2):31-36.

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  • 在线发布日期: 2013-06-16
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