(延边大学 医学院, 吉林 延吉 133002)
(College of Medicine, Yanbian University, Yanji 133002, China)
研究榆干离褶伞溶栓酶(Lyophyllum ulmarium fibrinolytic enzyme,LUFE)对高脂血症大鼠血管内皮损伤的保护作用。将SD大鼠分为正常对照组、模型组、阳性对照组、LUFE组,正常对照组喂养普通饲料,其余组给予高脂饲料,其中LUFE组同时灌胃LUFE (400mg·kg-1·d-1),阳性对照组于饮食诱导第5周开始灌胃阿托伐他汀钙片(5mg·kg-1·d-1),分别于诱导4、8周后进行指标检测。采用苏木精-伊红染色观察主动脉组织病理学形态变化,油红O染色观察主动脉内膜脂质斑块的分布情况。检测血清中总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、过氧化氢酶(CAT)、丙二醛(MDA)、超氧化物歧化酶2(SOD2)、内皮素-1(ET-1)、6-酮前列腺素F1α(6-keta-PGF1α)水平,并计算动脉粥样硬化指数。采用Western blot法检测主动脉核因子E2相关因子2(Nrf2)、沉默信息调节因子3(Sirt3)、乙酰化超氧化物歧化酶2(Ac-SOD2)、血红素加氧酶-1(HO-1)的表达水平。结果表明:LUFE可减轻高脂血症大鼠主动脉病理性损伤,减少主动脉内膜脂质斑块分布的相对面积,降低血浆TC、TG、LDL-C、ET-1、MDA水平,增加6-keta-PGF1α、CAT和SOD2的水平,上调主动脉组织Nrf2、HO-1、Sirt3蛋白表达,降低主动脉组织Ac-SOD2的相对蛋白表达。研究结果证实,LUFE可减轻高脂血症所致的大鼠血管内皮损伤,其机制可能与降低血脂、减轻氧化应激损伤有关。
The protective effect of Lyophyllum ulmarium fibrinolytic enzyme (LUFE) on vascular endothelium injury in hyperlipidemic rats was investigated. The SD rats were randomly divided into normal control group, model group, positive control group, and LUFE group. The normal control group was fed with normal diet, while the rest groups were given high-fat diet, in which the LUFE group was simultaneously gavaged with LUFE (400mg·kg-1·d-1). Positive control group started gavage of atorvastatin calcium tablets (5mg·kg-1·d-1) at the 5 week of feeding. After 4 weeks and 8 weeks of rearing, the corresponding indexes were tested respectively. Hematoxylin-eosin staining was used to observe the pathological changes of aorta. Oil red O staining was used to observe the distribution of lipid plaques in aortic intima. The levels of plasma total cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), catalase (CAT),malondialdehyde (MDA), superoxide dismutase 2 (SOD2), endothelin-1 (ET-1) and 6-keta-PGF1α were measured and the atherogenic index was calculated. Nuclear factor erythroid 2-related factor (Nrf2), silent information regulator 3(Sirt3), acetyl-superoxide dismutase 2(Ac-SOD2), and heme oxygenase 1(HO-1)of aorta were detected by Western blot. LUFE attenuated the pathological injury of aorta in hyperlipidemic rats, reduced the relative area of lipid plaque distribution in aortic intima, decreased the levels of TC, TG, LDL-C, ET-1 and MDA in plasma, and increased the levels of 6-keta-PGF1α, CAT and SOD2. The expression of Nrf2, HO-1 and Sirt3 protein in aortic tissue was up-regulated, and relative protein expression of Ac-SOD2 in aortic tissues was reduced. LUFE attenuated vascular endothelium injury induced by hyperlipidemia in rats, and the mechanism may be related to the reduction of blood lipid and oxidative stress injury.