高效液相色谱-氢化物发生原子荧光光谱法测定食品中多形态硒含量
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(1.谱尼测试集团股份有限公司, 北京 100095;2.谱尼测试集团北京科学技术研究院有限公司, 北京 100095)

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Determination of Content of Polymorphic Selenium in Foods by HPLC-HG-AFS
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( 1.Pony Testing International Group Co Ltd, Beijing 100095, China;2.Pony Testing International Group Beijing Science and Technology Research Institute, Beijing 100095, China)

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    摘要:

    研究基于高效液相色谱-氢化物发生原子荧光光谱建立了一种适用于富硒蛋白粉、富硒大米、富硒香菇和富硒酵母等富硒食品中硒酸根、硒代胱氨酸、甲基硒代半胱氨酸、硒代蛋氨酸的检测方法。样品经Tris缓冲液提取,蛋白酶和胰蛋白酶酶解,以V(20mmol/L磷酸氢二胺水溶液,pH=6.0)∶V(甲醇)=98∶2为流动相,经Hamilton PRP-X100阴离子交换色谱柱分离测定。结果表明,4种硒形态化合物在5~100μg/kg范围内线性良好(R2>0.999),方法检出限可达25~50μg/kg,平均加标回收率为81.37%~93.80%,且RSD为2.80%~7.22%。应用此方法对4种市售富硒食品(富硒蛋白粉、富硒大米、富硒香菇和富硒酵母)中4种硒形态化合物进行检测,前处理步骤简单,测定快速、准确度和灵敏度高,为食品中硒形态评价和质量控制提供了方法和依据。

    Abstract:

    A high performance liquid chromatography-hydride generation-atomic fluorescence spectrometry (HPLC-HG-AFS) for determination of selenium speciations (Se(VI), SeCys2, SeMeCys and SeMet) in Se-enriched foods (Se-enriched protein powder, Se-enriched rice, Se-enriched shiitake mushrooms and Se-enriched yeast, etc.) was developed in this study. Samples were extracted with Tris buffer and hydrolyzed by protease and trypsin, followed by separating on Hamilton PRP-X100 anion exchange column with a mobile phase consisting with V (20mmol/L diamine hydrogen phosphate aqueous solution,pH=6.0)∶V (methanol)=98∶2. Four speciations of selenium were completely separated as achieving the linearity larger than 0.999, the method detection limit reached 25-50μg/kg, and the average recovery rate was 81.37%-93.80% (RSD 2.80%-7.22%). This method was used to detect 4 kinds of selenium morphological compounds in 4 kinds of commercially available selenium-rich foods (Se-enriched protein powder, Se-enriched rice, Se-enriched shiitake mushrooms and Se-enriched yeast). The advantages of this method were simple pretreatment procedure, rapid determination, high accuracy and sensitivity, which provided a method and basis for the evaluation and quality control of selenium in food.

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房艳,王贝,高俊海,张雅莉.高效液相色谱-氢化物发生原子荧光光谱法测定食品中多形态硒含量[J].食品科学技术学报,2020,38(6):69-75.

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  • 收稿日期:2019-07-26
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  • 在线发布日期: 2020-12-28
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