The cartons diamond packaging market becomes more and more popular, but carton products by their own limitations (such as going out to carry, improper preservation, etc.) is prone to the deformation and damage. To enhance the image of the terminal product,and provide consumers with more convenient products, high density polyethylene (HDPE ) bottles for yogurt at room temperature were studied. In this study, the feasibility and optimal parameters of the secondary sterilization of the room temperature yoghurt in high density polyethylene bottle were studied. The sensory evaluation, viscosity, and microbial test results of the yogurt were determined by the L₉(3⁴) orthogonal test with the temperature and time as factors. The results showed that the optimal parameter for HDPE bottled yogurt was the 75℃, 20min secondary sterilization and the shelf-life of yogurt was 90 days.