In order to improve the methane production in the process of soybean wastewater degradation, effects of the trace elements,fermentation temperature, organic loading rate, and sludge concentration on the amount of methane produced by anaerobic fermentation of soybean wastewater were studied by the orthogonal experiment. Meanwhile, the effecting order of the factors and optimal technological conditions were confirmed. The results showed that the factors which had the greatest influence on the methane production were organic loading rate, followed by the addition of trace elements and sludge concentration, and the fermentation temperature had the least effect. The optimized combination of technological conditions was as follows:1mg/L Co, fermentation temperature 45℃, organic loading rate 1.52kg COD/(m³·d), and the sludge concentration was 30.00g VSS/L. Under this condition, the cumulative methane production was up to 970mL, which was increased about 15.10%. While the cumulative methane yield, the methane content in the biogas,and the removal rate of soluble chemical oxygen demand (SCOD) were 344.49mL/(g SCOD), 84.12%, and 84.00%, respectively.