Dendrobium officinale is one of the valuable Chinese herbal medicines which is rich in nutrients. In this study, the contents of water, soluble protein, total protein, crude fiber, fat, total sugar, mineral and amino acids in stems and leaves of D. officinale for two seasons were determined. The results showed that the contents of water (863.10mg/g), polysaccharide (289.90mg/g), iron (71.10mg/kg), and selenium (1.07mg/kg) were much higher than that of stems in autumn. Compared with stems, the content of polysaccharides in leaves was lower (the autumn leaf content was 162.30mg/g), but there were a higher soluble protein (4.20mg/g), total protein (67.03mg/g), crude fiber (204.60mg/g) and fat (14.70mg/g) in leaves. In addition, the content of amino acids in leaves was significantly higher than that in stems, and aspartic acid and glutamic acid contents were the highest, reaching to 13.60mg/g and 13.80mg/g, respectively. So the leaves had a certain nutrition value. Therefore, D. officinale can be used as a medicinal material for further research and development.