To address the problem of low bioavailability of curcumin due to poor water solubility and environmental sensitivity, an oil-in-water bilayer emulsion delivery system was constructed to improve the stability and bioaccessibility of curcumin. The bilayer emulsion was prepared by layer-by-layer electrostatic deposition technique using lactoferrin-epigallocatechin gallate (LF-EGCG) conjugates as the primary emulsifier and sodium alginate (SA) as the outer material. After optimizing the pH value and SA addition concentration, the particle size, Zeta-potential, rheological properties and environmental stability of the emulsion were analyzed. Then, the bilayer emulsion loaded with curcumin was prepared and the effects of the emulsion system on the encapsulation efficiency, environmental stability and digestive properties of curcumin were evaluated. The results showed that at pH 4.0, a stable bilayer emulsion (LF-EGCG/SA) with particle size of (949.06±85.81)nm and Zeta-potential of(-40.93±1.50)mV could be formed by secondary shear and high-pressure homogenization at SA mass concentration of 0.8%. The addition of SA could significantly improve the apparent viscosity and freeze-thaw stability of the emulsion, and reduce the sensitivity of the emulsion to variations in ionic strength and pH. At pH 4.0, curcumin encapsulation in the LF-EGCG/SA bilayer emulsion reached 96.66%±0.29%, and the bilayer emulsion prepared at pH 4.0 exhibited a higher residual curcumin level than that in monolayer emulsion after light, heat and storage treatment. After simulated digestion, the bilayer emulsion at pH 4.0 showed a free fatty acid release rate of 55.85%±0.21% and a bioaccessibility of 43.79%±1.23%. The bilayer emulsion constructed in the study could effectively protect and deliver curcumin, improve its environmental stability and digestive properties significantly, and provide a new perspective for the stable delivery of hydrophobic bioactive substances. 〖