In order to develop natural bio-based composite film materials with good preservation effects, bacterial cellulose (BC) and chitosan (CS), which have similar structural and functional properties, were used as the basis to composite cyclodextrin-resveratrol inclusion compound (RES), and finally bacterial cellulose-chitosan/cyclodextrin-resveratrol inclusion composite film (BC-CS/RESn) was obtained. The effects of RES addition (0,5%, 10%, 15%, 20%) on the physicochemical and functional properties of BC-CS/RESn were investigated. The Fourier transform infrared spectroscopy and X-ray diffraction results showed that there were non-covalent interactions such as hydrogen bonding and van der Waals forces between RES and BC-CS, and the addition of RES improved the mechanical and barrier properties of the composite films. The tensile strength of the composite film was (81.64±0.14) MPa, and the elongation at break was (27.83±3.40) % when the addition of RES was 10%. The oxygen permeability of the BC-CS/RES₃ could reach (0.85±0.03) g/(m²·s) when the addition of RES was 15%. The water vapor permeability of the BC-CS/RES₄ was the best at 20% RES addition, which was (6.74±0.02) g/(m²·24h). The addition of RES gave the composite film good antioxidant and bacteriostatic properties, in which the DPPH radical scavenging capacity of the composite film with 20% RES addition increased by 13.31 times after 24h, and the ferric iron reduction capacity increased by 49.99%, and the antibacterial ability of the composite film against Escherichia coli and Staphylococcus aureus increased by 4.65 times and 2.42 times, respectively. When the composite film was applied to the preservation of refrigerated fish fillets, the accumulation of volatile basic nitrogen during storage was effectively delayed. Therefore, the BC-CS/RESn had potential application value in aquatic preservation.