In this study, the physicochemical properties, component contents, microstructure and sensory qualities of the skeleton pulp of Lateolabrax japonicus obtained from different degrees of wet superfine grinding were analyzed. The physicochemical properties of fish skeleton pulp were measured using pH meter, ultraviolet spectrophotometer, colorimeter, texture analyzer, and laser particle size analyzer. Automatic amino acid analyzer was employed to detect the varieties and contents of amino acids. The microstructure was observed by scanning electron microscope. The results showed that, the particle sizes of samples larger than 100 mesh size were almost ranged from 50 to 100μm, while the particle sizes of samples less than or equal to 100 mesh size were mainly distributed between 100 and 300μm after wet superfine grinding at different degrees. In addition, pH value, ultraviolet absorbance, whiteness and bulk density were increased gradually with the increasing degree of wet superfine grinding. While the gel strength was first increased and then decreased. When the grinding degree increased from 20 mesh to more than 180 mesh, the content of calcium dissolution was increased from 151.23mg/kg to 270.06mg/kg, the collagen content was also slowly increased from 0.01% to 0.04%, the total content of amino acids was increased from 181.07ng/μL to 462.70ng/μL, the content of salt-soluble protein was increased from 6.95mg/g to 16.12mg/g,and the content of water-soluble protein was gradually increased from 2.15mg/g to 7.13mg/g. Furthermore, the scanning electron microscopy results revealed that samples with particles larger than 100 mesh were relatively smooth and uniform, while fish bones could be obviously observed in samples smaller than or equal to 100 mesh. The content of volatile basic nitrogen was in accordance with the relevant national standards. Combined with sensory evaluation, fish skeleton pulp above 100 mesh could be used as ingredients for fish powder, fish balls and other products, while fish skeleton pulp of less than or equal to 100 mesh might only be suitable for adding to products with large raw materials particle size such as soup packets, sauces and so on.