A HPLC-MS/MS method was established for simultaneous determination of nine sweeteners in foods (acesulfame K, saccharin sodium, sodium cyclamate, aspartame, sucralose, alitame, stevioside, advantame and neotame). After ultrasound and centrifugation, the samples were filtered through 0.22μm membrane and then separated with AQ-C18 column (100mm×2.1 mm×3μm) using 20mmol/L ammonium acetate solution and 0.1% formic acid acetonitrile solution as mobile phase and gradient elution were carried out. Under ESI negative ion mode,multiple reaction monitoring mode was used to qualitatively determine the mass number, retention time, and quantitatively measure the peak area. The results demonstrated that the calibration curves of nine sweeteners were good linearity for sweeteners with correlation coefficients of 0.9990. The limits of quantification of the nine sweeteners were 0.020-0.055mg/kg. The adding standard recovery rate was 90.6%-111.5%, and the relative standard deviation was less than 6.0% (n=6). This method had the advantages including simple pretreatment, fast measurement and high accuracy and sensitivity, which was suitable for qualitative and quantitative measurement of 9 sweeteners in food(beverages, yogurt, jelly, candies and wines), and also met and greatly improved the requirements of national standard.