The jujube is easy to cause mildew due to its high water and sugar content. In this study, the Lingwu long jujube was used as the raw material, and the sterilizing rate as the response value. Based on the single factor tests, three main factors (concentrations of ε-polylysine hydrochloride, pasteurization temperatures, pasteurization time) affecting the sterilizing rate were screened from five factors (concentrations of ε-polylysine hydrochloride, sterilization methods, pasteurization temperatures, pasteurization time, packing methods) by Plackett-Burman design test. The quadratic multiple regression equation model of each factor on sterilizing rate optimizing by Box-Behnken design test was established. The results showed that the best quality assurance technology was as follows:the concentrations of ε-polylysine hydrochloride was 0.08g/kg, the fixed sterilization method was pasteurization at temperature of 75℃ and last 40min, the packaging method was nitrogen-filled packaging, and the sterilizing rate was 98.00%. It suggested that the optimized quality assurance technology could make dry jujube as exempt washes jujube, delay the growth of microorganisms, and make the total number of bacteria reach the standard of exempt washes jujube, which provided a theoretical basis for the industrialization development of exempt washes jujube.