In order to shorten the reaction time and improve the stability of Channa argus short peptide-zinc (CASP-Zn), the ultrasonic technology was employed to analyze the effects of different time (0,5, 10,5, 30,5, 60,5, 90,0min) and different ultrasonic power (0,0, 240,0, 480W) on the chelating rate, spectral structure, microstructure and antioxidant activity of CASP-Zn. The results showed that the ultrasonic treatment significantly affected the chelating rate of CASP-Zn , and the chelating rate of CASP-Zn was the strongest and around 93.0% after ultrasonic treatment with the power of 360W. In a short time (15min), the chelating rate of CASP-Zn after ultrasonic treatment with different power reached a higher value. Compared with other ultrasonic treatment groups, the maximum UV-VIS absorption peak and fluorescence spectrum intensity of CASP-Zn treated with the power of 360W were the highest. Scanning electron micrograph analysis showed that the ultrasonic treatment group of 360W had the closest combination. CASP-Zn also had certain antioxidant activity. Among them, CASP-Zn treated with different ultrasonic power had no significant influence on superoxide anion free radical and H₂O₂ scavenging rate, while CASP-Zn after ultrasonic treatment with power of 360W had a strong DPPH free radical scavenging rate. It showed that the antioxidant activity was positively correlated with the stability of chelate complex. Ultrasonic technology could promote the chelation reaction and enhance the stability and antioxidant activity of peptide-zinc complex.