南、北山楂提取液分别对人体内源淀粉酶作用研究
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(广西中医药大学 药学院, 广西 南宁 530200)

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广西教育厅中青年教师基础能力提升项目 (2017KY0287;2018KY0272);广西科技计划项目(AD18281081;2018GXNSFAA294078);广西中医药大学博士科研启动项目(2017BS030);福州市科技计划项目(2017- G- 60);广西中医药大学校级项目(XCS17005;XP017326)。


Effect of Nippon Hawthorn and Chinese Hawthorn Extract on Human Endogenous Amylase Activity
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(College of Pharmacy, Guangxi University of Chinese Medicine, Nanning 530200, China)

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Guangxi Education Department Basic Ability Improvement Project for Young and Middle-aged Teachers (2017KY0287; 2018KY0272); Guangxi Science and Technology Project (AD18281081; 2018GXNSFAA294078); Doctoral Research Project of Guangxi University of Traditional Chinese Medicine (2017BS030); Fuzhou Science and Technology Plan Project (2017-G-60); University-Level Project of Guangxi University of Traditional Chinese Medicine(XCS17005; XP017326).

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    摘要:

    通过测定淀粉酶与山楂提取液反应后的酶活,分别探讨南、北山楂是否可以通过提高淀粉酶的活性来达到帮助消化的效果。在不同温度(30、60、80℃)下分别制备南、北山楂浸提液后,模拟人体肠道环境,分别考察在不同的反应温度(25、37、45℃)、不同的pH值(6.5、6.8)和离子条件(C2O2-4、Zn2+)下,南、北山楂水提液对淀粉酶活性的影响。结果发现,在所考察的反应温度范围内和pH值条件下,南、北山楂水提液在一定程度上均可以提高淀粉酶活性。其中,温度37℃、pH值为6.5的条件下,60℃制备的北山楂水提液对淀粉酶活性的促进作用最强,C2O2-4对淀粉酶与北山楂反应的影响呈现促进作用,而Zn2+对淀粉酶与南、北山楂的反应均表现为抑制作用。

    Abstract:

    In this study, the activity of amylase was measured to discuss whether Nippon hawthorn (Crataegus cuneata) and Chinese hawthorn (Crataegus pinnatifida) could promote digestion by improving the activity of amylase. After the preparation of hawthorn extracts at different temperatures (30,0, 80℃), the intestinal environment was simulated and the effects of hawthorn water extracts on amylase were investigated at different reaction temperatures (25,7, 45℃), different pH (6.5,6.8), and ion conditions (C2O2-4, Zn2+). The results demonstrated that the activity of amylase could be increased by hawthorn extracts at each reaction temperature and pH. Among them, the extracts from Chinese hawthorn under 60℃ showed the strongest promoting effect on activity of amylase under the reaction conditions of 37℃ and pH 6.5. C2O2-4 had the promoting effect on the reaction of amylase with the Chinese hawthorn, while Zn2+ showed the inhibitory effect of extracts from Nippon hawthorn and Chinese hawthorn on promotion of amylase activity.

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祁静,杨娟,汪启珍,蔡炳民,于迪,孙一铭.南、北山楂提取液分别对人体内源淀粉酶作用研究[J].食品科学技术学报,2020,38(1):80-87.

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  • 收稿日期:2019-04-08
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  • 在线发布日期: 2020-01-21
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