Low-methoxy pectin/chitosan composite membranes were prepared through the tape casting method. The formation mechanism of the composite membranes and effects of pectin, chitosan, and glycerol on the structure and properties of the membranes were discussed.The results showed that —COOH in pectin bounded to —NH₂ in chitosan and promoted the formation of the composite membranes. The tensile strength and elongation at break of the composite membranes were increased firstly and then decreased with the increasing amount of pectin. And the composite membranes reached their maximum tensile strength (4.12MPa) and elongation at break (33.24%) when the addition of pectin was 4g. With the increasing chitosan addition, the elongation at break of the composites membranes was decreased, while the tensile strength was firstly increased and then decreased. The composites membranes attained its maximum tensile strength (4.13MPa)when the addition of chitosan was 4g. When the glycerol addition was 1mL, the composites membranes got a great tensile strength of 24.32MPa, with a poor elongation at break (4.86%). With the increase of glycerol addition, the tensile strength of the composites membranes was sharply decreased, while the elongation at break was rapidly increased.