The immobilization methods using sodium alginate as the embedding medium and flutaraldehyde as the crosslinker was used to improve the cyclic utilization and stability of immobilized sucrose isomerase PalI from Klebsiella sp. LX3.The results showed that the highest enzyme activity was obatained when the concentrations of sodium alginate and CaCl₂ were 1.5% and 2%. Meanwhile, the optimum temperature and pH value of embedding enzyme were 40℃ and 6.0. The optimum condition of the crosslinking method was the concentrations of ammonium sulfate and flutaraldehyde were 90% and 2.5%. Meanwhile, the optimum temperature and pH value of crosslinking enzyme were 50℃ and 5.0. Through the comparison of stability, the conclusion was that the enzyme activity of both methods was better than that of free enzyme. The residual enzyme activity of immobilized enzyme with flutaraldehyde and sodium alginate kept in 95% and 60%, while that of the free enzyme was only 30% when enzymes were stored at 4℃ for 20 days. The crosslinking immobilization enzyme was superior to the sodium alginate immobilized enzyme, and its residual enzyme activity was still 80% after repeated use for 12 times.