Ten strains were isolated from infected grapes and grape leaves in the suburb of Beijing and analysis the pathogenicity of different pathogenic bacteria to four fruits. In order to provide reference for the study of interspecific relationship and disease control of fungi. Three strains 814#, 815# and 880# were isolated from infected grapes. Seven strains 794#, 797#, 798#, 811#, 902#, 903# and 1397# were isolated from infected grape leaves. The fungal pathogens 814#, 815#, 880#, 794#, 797#, 798#, 811#, 902#, 903# and 1397# were identified as Phoma sp., Aspergillus aculeatus, Botrytis cinerea, Aspergillus japonicas,Trichothecium roseum, Botrytis porri, Colletotrichum fructicola, Alternaria eichhorniae, Fusarium redolens and Neurospora terricola by morphological characterization and rDNA ITS sequence analysis. In addition, A. aculeatus was firstly isolated from grapes and A. japonicas, C. fructicola, A. eichhorniae, F. redolens and N. terricola were firstly isolated from grape leaves. Reinoculated fruits such as grapes, cherry tomatoes, apples and pears showed that B. cinerea, A. japonicus, A. aculeatus, T. roseum, B. porri, C. fructicola, A. eichhorniae and F. redolens can cause all the fruits disease and B. cinerea, A. japonicus, B. porri and F. redolens showed strong pathogenicity. In contract, Phoma sp. and N. terricola can only cause grapes and cherry tomatoes disease.