Human milk provides the optional balanced nutrition for the growing infant after birth. In recent years, researchers are gradually aware that the specific esterification position of palmitic acid in human milk (about 70% of palmitic acid esterificated in the Sn-2 position of triglycerides) has an important role in promoting infant health. With human milk as the gold standard, the nutritional and physiolo-gical functions of infant formula can be significantly improved by mimicking the proportion of Sn-2 palmitate of human milk in infant formula. This paper reviews the important role of Sn-2 palmitate for infant health, such as benefiting fatty acids and minerals absorption, bone growth rate, mineralization and strength, intestinal flora, infant crying, and brain development.