Cronobacter spp. is a group of foodborne pathogens causing serious infections including neonatal meningitis, necrotizing enterocolitis and bacteremia with high fatality rate. In this study, 120 dry seafood samples were collected to study the contamination investigation of Cronobacter spp. and further study on biological characterization was performed. The detection rate of Cronobacter spp. was 17.83%. Then, the relationship between biofilm forming abilities and motility of 23 isolates were explored. Meanwhile, the No.11 strain had the strongest biofilm-forming ability and relatively stronger motility than most of the isolates. Results showed that the contamination rate of Cronobacter spp. in dry aquatic food was high and different strains had different biofilm forming abilities and motilities, which provided important basic data for the prevention and control of Cronobacter spp. in food production.