The study established a simple and rapid method for the determination of Bacillus cereus. According to the 16S RNA gene sequences of Bacillus cereus available in Genbank, specific primers and exo probe were designed for establishing real-time recombinase polymerase amplification(real-time RPA).The RPA reaction was performed successfully at 39℃ and the results were obtained within 20min. This method could specifically detect Bacillus cereus, but could not detect other bacteria. The study showed that the detection limit of real-time RPA was 1.0×10-3ng/μL with genomic DNA of Bacillus cereus, which was the same as the real-time PCR method. Bacillus cereus in artificially contaminated rice samples with a bacterial concentration of 1.5×10⁴CFU/g could be detected after 6-13min by real-time RPA; when the bacterial concentration was 1.5×10⁵CFU/g, the Bacillus cereus could be detected at least 30 min by real-time PCR (the Ct value was between 20 and 31).