Total volatile basic nitrogen (TVB-N) content is an important indicator for pork freshness detection. In order to detect the concentration of hydrogen sulfide gas during the deterioration of pork, tunable diode laser absorption spectroscopy (TDLAS) was designed. Meanwhile, the content of TVB-N in the same environment was measured using the GB method. And the relationship between the concentrations of TVB-N and H₂S in the corruption process of fresh pork at 25℃ and 70% relative humidity (RH) was explored. The results showed the contents of TVB-N and H₂S in the fresh pork gradually increased, and the H₂S concentration was positively correlated with the TVB-N content at different time points (p<0.02). Therefore, it is possible to measure the TVB-N content indirectly by TDLAS detection in the process of pork degeneration. The TVB-N content can be obtained quickly with high accuracy and the efficiency of meat detection is improved.