This study assessed the proliferation factors and culture conditions for the growth of a Saccharomyces cerevisiae TR2 isolated from Xinjiang traditional fermented camel milk and established its kinetic model, which could reflect the high density fermentation and the application of Saccharomyces cerevisiae in milk beer production. The carbon source, nitrogen source, inorganic salts, species and concentrations of the growth factors in culture medium were screened by the single factor test. Meanwhile, the compositions of culture medium and fermentation conditions of Saccharomyces cerevisiae were optimized by response surface and orthogonal methods. Moreover, the strain growth and the kinetic model of glucose consumption were fitted nonlinearity by Matlab software. The results showed that the optimal medium was glucose 53.4g/L, peptone 21.5g/L, yeast extract 10g/L, and KH₂PO₄ 2.98g/L. Under the fermentation conditions of initial pH 5, shaking speed at 150r/min, inoculum concentration 5%, liquid volume 60mL, incubation time 16h and incubation temperature 28℃, the cell number reached to 6.64×10⁸CFU/mL. The predictive value of the kinetic model for the fermentation process was well fitted to the experimental value, and the fitting degrees were 0.9973 and 0.9784. It concluded that the model could reflect the fermentation process of the strain in batches.