To identify Leuconostoc mesenteroides BD3749 at the subspecies level and to explore the application prospect of this strain in food industry, BD3749 was identified at the subspecies level by molecular biology and biochemistry techniques. The growth of BD3749 in tomato juice supplemented with sucrose was investigated through dynamic monitoring of OD₅₉₅ and pH as well as the remnant amount of sucrose. The productivity of exopolysaccharide (EPS) was determined to evaluate the potential of BD3749 to serve as dietary supplement. The effect of oxygen stress on the amount of EPS was analyzed and the growth condition was optimized accordingly for EPS production. BD3749 was assigned to be L. mesenteroides subsp. mesenteroides based on these studies. Furthermore, BD3749 grew well in tomato juice supplemented with sucrose, and EPS produced was mainly insoluble EPS (iEPS). Subsequently, the effect of oxygen on EPS production was analyzed and the condition was optimized accordingly for EPS production. In the optimized content of oxygen, 14.5g/L iEPS was achieved after 48h fermentation. BD3749 is a strain of L. mesenteroides subsp. mesenteroides, which is generally regarded as safe and can be applied in food industry. With the excellent fermentation performance, BD3749 is a strain of high potential in food industry.