In order to improve the extraction rate of peanut oil bodies, Viscozyme L. was used to assist the extraction of peanut oleosome. The effects of material ratio, enzyme concentration, incubation time, and incubation temperature on extraction rate of peanut oil body with compound plant hydrolase were investigated by using the single factor experiment. On the basis of single factor experiment, the orthogonal experiment was used to optimize the process conditions of extracting peanut oil body with compound plant hydrolase. The results showed that the influence order from high to low of the factors was material ratio, incubation temperature, incubation time, enzyme concentration. The optimum conditions were as follows:material ratio 1∶4 (g/mL), enzyme concentration 1.25%, the incubation time 80min and the incubation temperature 50℃. Under the optimum conditions, the extraction rate of peanut oil body was 48.92%.