食用植物油中生育酚含量测定方法优化及分析
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(北京工商大学 食品学院/食品添加剂与配料北京高校工程研究中心, 北京 100048)

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李 柰,女,硕士研究生,研究方向为生物分离工程; *曹学丽,女,教授,博士,主要从事生物分离技术方面的研究。通信作者。

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北京市自然科学基金重点资助项目(KZ201410011016)。


Optimization of Determination Method and Analysis on Tocopherol Content in Edible Vegetable Oils
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(School of Food and Chemical Engineering/Beijing Higher Institution Engineering Research Center of Food Additives and Ingredients, Beijing Technology and Business University, Beijing 100048, China)

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    摘要:

    采用高效液相色谱法测定了8种24个植物油样本中生育酚的含量,并对生育酚的提取条件进行优化。在室温下,以甲醇为溶剂,采用超声法进行提取,料液比为1∶1.25,提取3次,每次20min,采用液相色谱方法进行分析。结果表明:该方法在生育酚质量浓度为0.05~1.00g/L时,相关系数R2可达到0.9992~0.9994,线性关系良好;检出限分别为α-生育酚0.5mg/L,γ-生育酚2.0mg/L,δ-生育酚1.2mg/L;三水平加标样品的平均回收率为92.1%~99.8%,相对标准偏差(RSD,n=3)为2.5%~8.5%。在8种植物油中,葵花籽油中α-生育酚的质量分数较高,可达4.2349×10-4,大豆油的β/γ-生育酚、δ-生育酚及总生育酚的质量分数较高,分别为7.3713×10-4,1.6215×10-4,9.8836×10-4。

    Abstract:

    The tocopherol contents in 8 kinds of edible vegetable oils (total 24 samples) were determined by HPLC and the extraction conditions were optimized. Tocopherol was extracted three times by the ultrasonic method and the extraction conditions were liquid material ratio 1∶1.25 and extraction time 20min. The results showed that the correlation coefficient R2 was 0.9992-0.9994 within the scope of 0.05-1.00g/L. The detection limits of α-tocopherol, γ-tocopherol, and δ-tocopherol were 0.5,2.0,1.2mg/L, respectively, and the recovery average was 92.1%-99.8%. Meanwhile, the relative standard deviation (RSD, n=3) was between 2.5%-8.5%. Sunflower seed oil had the highest α-tocopherol content (4.2349×10-4) while soybean oil had the highest β/γ-tocopherol (7.3713×10-4), δ-tocopherol (1.6215×10-4), and total tocopherols (9.8836×10-4) contents.

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李 柰,樊 琛,李小波,龙立梅,曹学丽.食用植物油中生育酚含量测定方法优化及分析[J].食品科学技术学报,2015,33(3):59-63, 69.

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  • 在线发布日期: 2015-05-29
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