Chitosan, sodium alginate, activated carbon,and graphene oxide were selected to immobilizenaringinase. By comparing the activities of immobilized naringinase and the enzyme-carrying rates of the enzyme carriers, it was found that graphene oxide was the optimal material. The enzyme-carrying rate of graphene oxide was 85.61% and theenzyme activity of graphene oxide immobilized naringinase was 410.50U/g. The enzyme activity remained 72.38% relative activity after 7 times using. Simultaneously, the effects of different conditions on catalytic activity of graphene oxide immobilized naringinase were studied. It was obtained that the optimal load amount of naringinase per gram of graphene oxide 60mg, optimal temperature 20℃, optimal pH 4.0, and immobilization adsorption time 9h. The activity of graphene oxide immobilized naringinase reached 433.70U/g under the optimized conditions, which remained 78.62% relativity activity after 7 times using.