The diversity of culturable endophytes in wild Noni leaves from Paracel Islands was discussed in this study. Strains were isolated by using conventional tablet separation methods. The isolates were identified by the fungal internal transcribed spacer 1 and 2 (ITS gene) amplification and the bacterial 16S rDNA amplification, combined with phylogenetic analysis. Total 32 endophytes were isolated and identified as 19 types. Two types of mold were Chaetomium sp. and Nemania sp. Seventeen types of bacteria (30 strains) belonged to 7 genera Bacillus sp., which were Lysinibacillus sp., Micrococcus sp., Terribacillus sp., Enterobacter sp., Xanthomonas sp., Flavobacterium sp., and Bacillus sp.. Bacillus sp. was the dominant genus among the endophytic bacte, which had 20 strains divided into 11 types.