Capsanthin and capsaicine were extracted from several different chili using Soxhlet extraction, and the optimum extraction condition were studied. The resine adsorption and solvent liquid extracting were used to separate the capsanthin from capsaicine. The results showed that the content of red pigment in Millet pepper was the highest, the optimal extraction solvent was chloroform, and extraction time was 50min. XAD16 adsorption resin could adsorb capsanthin dissolved in ethanol, the saturation adsorption of 1g resin was 0.017g capsanthin,. The optimum extracting conditions of capsanthin were as follows: 80% ethanol, 3 times extraction, extraction for 60min. In addition, alkalescence metal oxid could eliminate capsaicin very well.