高效降解废弃蓖麻基润滑油降解菌的分离筛选 及特性研究
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Isolation of Castor Oil-based Waste Degrading Bacteria and Its Degradation Characteristics
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    摘要:

    从内蒙古某蓖麻榨油厂排污口采样,分离筛选出10株能降解废弃蓖麻基润滑油菌株,其中T9菌株降解润滑油的能力较强,该菌株最适降解pH值为5.0,降解温度30℃,在1%~5%的NaCl中能较好生长。 通过菌落形态与生理生化实验,初步鉴定该菌株为假单胞菌属(Pseudomonas) 。 在润滑油质量浓度为10 g/L, 初始pH值为5.0, 180r/min, 30℃下培养7d后,采用改进的CECL33A93方法测得其对废弃蓖麻基润滑油的降解率为72%。 采用GC/MS对降解产物进行分析,测得其对废弃蓖麻基润滑油降解率为80%,该菌株具有良好的蓖麻基润滑油降解能力。

    Abstract:

    Ten strains which can degrade the waste lubricants were isolated from sewage samples of the outfall of a castor oil extraction plant in Inner Mongolia, China, and T-9 strain showed the strongest lubricant degradation ability. The optimal degradation condition of T-9 strain was pH 5.0, and temperature 30℃. T-9 strain could grow well in the medium containing 1%~5% NaCl. T-9 strain was identified as Pseudomonas by the colony morphology, physiological and biochemical characteristics. By improved CEC-L-33-A-93 method, the degradation rate of castor oil-based waste lubricants was 72% when incubated for 7 days under the conditions as following: 10g/L oil, initial pH 5.0, rotation speed of 180r/min, and 30℃. By GC/MS analysis of degradation products, degradation rate was 80%, indicating that the T-9 strain had good castor-based oil degradation ability.

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刘亚琼,王昌禄,何东,张丽红,陈勉华,王玉荣,何良年,叶锋.高效降解废弃蓖麻基润滑油降解菌的分离筛选 及特性研究[J].食品科学技术学报,2011,29(6):15-19.

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  • 在线发布日期: 2011-12-12
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