A new edge extracting method of meat cells image based on Canny operator and mathematical morphology was established in this paper. The pork biopsy samples in different times during the natural storeage process were produced. First, the cell slice edge of 0 hour cell was extracted with the Canny operator, then structuring elements probe was used for dilation according to the mathematical morphology method, after that gaps within the edge of the fracture and the cell were filled, and smoothed. Experimental results show that this method is better than the Canny edge detection just based on the differential operator, which has laid a good foundation for the cell slice image feature extraction, recognition and others relative researches.