To study the anti-fatigue function and safety of Olea europaea extract compounded with L-carnitine tartrate, the mice were randomly divided into three dose groups (1.05,0.70,0.35g/kg) and one negative control group, and Olea europaea compounds were administrated by gavage. After 30 days, the weight loading swimming time, blood urea nitrogen, hepatic glycogen, and blood lactic acid were measured. The acute toxicity test, genotoxicity test and the 30 days feeding test in rats were used to evaluate the safety of Olea europaea compounds. The results showed that after gavage administration for 30 days, compared with the negative control group, the weight loading swimming time of mice significantly extended in high dose group (P<0.05). The blood urea nitrogen content in mice significantly decreased in high dose group (P<0.05) and extremely significantly decreased in middle dose group (P<0.01) after swimming 90min without weight loading. The accumulation of blood lactic acid in mice significantly decreased in high dose group (P<0.05) after swimming 10 minutes without weight loading. The content of hepatic glycogen in mice had no significant difference (P>0.05). The safety assessment results showed that the Olea europaea compounds had no acute toxicity or genotoxicity. Compared with the negative control group, the animal body weight, food intake, food utilization, hematology, blood biochemistry and histopathology in the three dose groups had no significant difference (P>0.05) during the 30 days feeding test. This indicated that the Olea europaea compounds had obvious anti-fatigue function and high safety.