A method for the determination of tartrazine, amaranth, ponceau, and sunset yellow in wines by high performance liquid chromatography -HPLC- was established. The separation was achieved on a ODS-C18 column by a gradient elution with methanol and 002mol/L aqueous ammonium acetate as mobile phase. The flow speed of the mobile phase was 08mL／min and the determination was conducted at 470nm through a UV detector. The average recovery of the method of real samples was more than 95%, and the RSD was less than 3%. Therefore, the method is suitable for the experiment.