The sheep waist was chosen as raw material for grease extraction in this paper. The effect of different solvents,and temperature on extraction of grease and conjugated linoleic acid-CLA- was studied. The results showed that hexane was the best solvent for extraction in comparison with petroleum ether, cyclohexane and ethyl alcohol. The optimum extraction conditions were determined by the orthogonal test as follows: temperature of 60℃, time of 4h, and V-liquid-∶m-solid-=8L/kg. Under the optimal conditions, the extraction rate of grease was 8872% and the content of CLA was 631mg/g. The content of CLA in grease decreased with the increasing temperature while increased slightly at 130℃.