To explore the regulation effects of micro-environment gas regulation on blueberry fruit softening during shelf life after controlled freezing-point storage, Legacy blueberry was harvested and stored in the micro-environment gas regulation box added 1-methylcyclopropylene preservation bag, shipped to the controlled freezing point room (-0.5℃±0.3℃) for 30d storage by cold chain logistics car and then commercially packed and storage at 7℃±1℃. Fruit firmness, cell wall polysaccharides content, cell wall degradation enzymes activity were determined to investigate the influence of micro-environment gas regulation on blueberry fruit firmness during shelf life and the correlation between blueberry firmness and cell wall metabolism indexes. The results showed that micro-environment gas regulation maintained higher contents of cellulose, hemicellulose and propectin in late stage of shelf life, delayed the increase of soluble pectin content, inhibited the activities of polygalacturonase, pectin methylesterases, α-L-arabinofuranosidase and cellulase, delayed the occurrence of β-galactosidase activity peak, and then delayed the decline of fruit firmness. Clustering and correlation analysis showed that the cellulose, hemicellulose, and pectin was divided into Ⅰ cluster. The correlation coefficient with fruit firmness was 0.580,0.663 and 0.645, respectively, which had very significant positive correlation. And soluble pectin, polygalacturonase, pectin methylesterase, α-L-arabinofuranosidase, cellulase and β-galactosidase were divided into Ⅱ cluster, and negatively correlated with fruit firmness. The combination of micro-environment gas regulation and controlled freezing point storage could delay the softening of blueberry fruit during shelf life, which would provide a theoretical basis for maintaining the blueberry firmness during shelf life.