During the storage period, Botrytis cinerea infection was usually occurred, which caused serious rot in fruits and vegetables. In this study, cherry tomatoes infected with Botrytis cinerea were used to simulate the rot that might occur during storage, and the inhibitory effect of exogenous methyl jasmonate (MeJA) and isoleucine (Ile) on the enzymes activity related to grey mold was studied. In the experiment, Ile group and MeJA+Ile group were first soaked in 10mmol/L Ile, and the MeJA group and MeJA+Ile group were subsequently fumigated with 10μmol/L MeJA at 30℃ for 48h, then them were infected with Botrytis cinerea spores, and stored at room temperature. And the lesion diameter and enzyme activity were measured. The results showed that cherry tomatoes treated with MeJA and/or Ile had higher enzyme activity in phenylalanin-eammonialyase, polyphenol oxidase, peroxidase, superoxide dismutase, ascorbate peroxidase and catalase after infection, which was significantly different with that of the control group (P<0.05). Compared with the other three groups, the enzyme activities measured in the MeJA+Ile group were all higher, while the lesion diameter was smaller, which indicated that cherry tomatoes with MeJA and Ile combined treatment could activate the disease resistance enzyme and enhance the resistance to the infection of Botrytis cinerea. This study provides a reference for the prevention of Botrytis cinerea induced rot in fruits and vegetables during postharvest storage.